Anti-Claudin 2 antibody ab53032 from Abcam

Product Specs
ItemAnti-Claudin 2 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab53032
Antibody TypePrimary
HostRabbit
ClonalityPolyclonal
Quantity100 UG
IsotypeIgG
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
Formatab53032 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Concentration1 mg/ml
Gene NameCLDN2
ReactivityHuman, Mouse
TargetClaudin 2

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Inhibitory effects of the nanoscale lysate derived from xenogenic dental pulp stem cells in lung cancer modelsJ NanobiotechnologyDecember 18, 2023Yan He, Ruohan Li, Wenting She, Yilong Ai, Kesheng Li, Tushar Kumeria, Ziran Jiang, Qing Shao, Chen Zou, Abdullkhaleg Ali Albashari, et al.anti-EDN1 (ab2786, 1:1000), anti-PI3K (ab278545, 1:1000), anti-CLDN1 (ab180158, 1:2000), anti-CLDN2 (ab53032, 1:500), anti-Caspase-3 (ab184787, 1:2000), anti-Bcl-2 (ab182858, 1:2000), anti-Bax (ab32503, 1:1000),

(2) Colonic phosphocholine is correlated with Candida tropicalis and promotes diarrhea and pathogen clearancenpj Biofilms and MicrobiomesSeptember 4, 2023Xihong Zhou, Yiwen He, Jingqing Chen, Xia Xiong, Jie Yin, Jing Liang, Can Peng, Chunxia Huang, Guiping Guan, Yulong YinCit-H3 (Cell Signaling Technology Cat#97272; RRID, not found; Dilution, 1:800), Claudin-2 (Abcam, Cat#ab53032; RRID:AB_869174; Dilution, 1:200), and Ki67 (Abcam Cat#ab15580, RRID:AB_443209; Dilution, 1:100) at

(3) Multidirectional characterization of cellular composition and spatial architecture in human multiple primary lung cancersCell Death & DiseaseJuly 25, 2023Yawei Wang, Di Chen, Yu Liu, Daiwang Shi, Chao Duan, Jinghan Li, Xiang Shi, Yong Zhang, Zhanwu Yu, Nan Sun, et al.serum for 30 min at 37 °C. The tissue microarrays were incubated with rabbit anti-CLDN2 (1:400, No.ab53032, Abcam) overnight at 4 °C. Then, the sections were incubated with biotinylated goat anti-rabbit IgG

(4) Alpha-tocopherylquinone differentially modulates claudins to enhance intestinal epithelial tight junction barrier via AhR and Nrf2 pathwaysCell reportsJuly 25, 2023Ganapathy AS, Saha K, Wang A, Arumugam P, Dharmaprakash V, Yochum G, Koltun W, Nighot M, Perdew G, Thompson TA, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Anti-Claudin-2,"Abcam, Boston, MA",Cat# Ab53032; RRID:AB_869174 Anti-Claudin-1,"Life Technologies, Carlsbad, CA",Cat# 37–4900; RRID:AB_2533323 Anti-Claudin-3,"Life Technologies

(5) Multiomics Strategy Reveals the Mechanism of Action and Ameliorating Effect of Deer Velvet Antler Water Extracts on DSS-Induced ColitisBiomedicinesJuly 6, 2023Ying-Kai Hung, Shang-Tse Ho, Ching-Yun Kuo, Ming-Ju ChenSDS-PAGE gels for analysis of the expression of occludin (ab216327), claudin1 (ab15098), claudin2 (ab53032), claudin4 (ab15104), Zonula occludens-1 (ZO-1) (ab96587), CCL-20 (ab9829), HIF-1α (ab2185), and β-actin

(6) Altered gut microbiota of obesity subjects promotes colorectal carcinogenesis in miceeBioMedicineJuly 1, 2023Xing Kang, Siu-Kin Ng, Changan Liu, Yufeng Lin, Yunfei Zhou, Thomas N.Y. Kwong, Yunbi Ni, Thomas Y.T. Lam, William K.K. Wu, Hong Wei, et al.PCNA(PC10) Mouse mAb (Cell Signaling Technology Cat# 2586, RRID:AB_2160343, 1:16000) or Claudin-2 antibody (Abcam Cat# ab53032, RRID:AB_869174, 5 μg/ml) at 4 °C overnight. Signals were developed with IHC Select DAB150 Immunoperoxidase

(7) Amino acid solution mitigates hypothermia response and intestinal damage following exertional heat stroke in male micePhysiological ReportsMay 22, 2023Michelle A. King, Astrid Grosche, Shauna M. Ward, Jermaine A. Ward, Anusree Sasidharan, Thomas A. Mayer, Mark L. Plamper, Xiaodong Xu, Matthew D. Ward, Thomas L. Clanton, et al.(clone MH25, RRID: AB_2533997; Invitrogen #71‐7800), claudin‐2 rabbit pAb (RRID: AB_869174; Abcam #ab53032), e‐cadherin rabbit mAb (clone 24E10, RRID: AB_2291471; Cell Signaling #3195), and claudin‐5 rabbit

(8) Ginkgo biloba Extract Preventively Intervenes in Citrobacter Rodentium-Induced Colitis in MiceNutrientsApril 21, 2023Chen T, Chen Y, Li K, Chen Z, Zhao Q, Fan Y, Liu Y, Zhang S, Hao Z.Proteintech Group, Inc., Wuhan, China), Claudin1 (13050-1-AP, Proteintech Group Inc., Wuhan, China), Claudin2 (ab53032, Abcam, Cambridge, UK), or Claudin3 (YT0949, Immunoway Biotechnology Co., Ltd, Plano, TX, USA) at 4

(9) DR3 Regulates Intestinal Epithelial Homeostasis and Regeneration After Intestinal Barrier InjuryCellular and Molecular Gastroenterology and HepatologyApril 1, 2023Yosuke Shimodaira, Shyam K. More, Hussein Hamade, Anna Y. Blackwood, Jay P. Abraham, Lisa S. Thomas, Jordan H. Miller, Dalton T. Stamps, Sofi L. Castanon, Noam Jacob, et al.Waltham, MA), anti–claudin-1 (1:200, cat. ab15098; Abcam, Waltham, MA), anti–claudin-2 (1:200, cat. ab53032; Abcam), and anti–occludin-1 (1:50, cat. 40-4700; Thermo Fisher Scientific). The following secondary

(10) P53 Deficiency Accelerate Esophageal Epithelium Intestinal Metaplasia MalignancyBiomedicinesMarch 13, 2023Qiu Q, Guo G, Guo X, Hu X, Yu T, Liu G, Zhang H, Chen Y, She J.Proteintech; Cat. No. 10831-1AP), OCLN (1:1000; ABCAM; Cat. No. ab31721), CLDN2 (1:1000; ABCAM; Cat. No. ab53032), P53 (1:1000; Proteintech; Cat. No. 60283-2-Ig), P21 (1:1000; Proteintech; Cat. No. 10355-1-AP), JAK1

(11) Heme-binding protein 1 delivered via pericyte-derived extracellular vesicles improves neurovascular regeneration in a mouse model of cavernous nerve injuryInternational journal of biological sciencesJanuary 1, 2023Ock J, Wu J, Liu FY, Fridayana FR, Niloofar L, Vo MN, Hong SS, Kang JH, Suh JK, Yin GN, et al.(Ca# NBP2-14977, 1:100; NOVUS Biologicals), claudin-1 (Ca# ab242370, 1:1000; Abcam), claudin-2 (Ca# ab53032, 1:1000; Abcam), claudin-3 (Ca# ab15102, 1:1000; Abcam), claudin-11 (Ca# 36-4500, 1:1000; Thermo Fisher

(12) Gut microbiota regulates acute myeloid leukaemia via alteration of intestinal barrier function mediated by butyrateNature CommunicationsDecember 1, 2022Ruiqing Wang, Xinyu Yang, Jinting Liu, Fang Zhong, Chen Zhang, Yuhong Chen, Tao Sun, Chunyan Ji, Daoxin Mafor immunohistochemistry. ZO-1 (Servicebio, GB111402,1:500), claudin-1 (Servicebio, GB11032,1:500) and claudin-2 (Abcam, ab53032,1:500) used for immunofluorescence. Secondary antibodies were obtained from ZSGB-BIO(China). All reagents

(13) Acteoside alleviates dextran sulphate sodium‑induced ulcerative colitis via regulation of the HO‑1/HMGB1 signaling pathwayMolecular medicine reportsDecember 1, 2022Guo W, Wang X, Liu F, Chen S, Wang S, Zhang Q, Yuan L, Du S.ProteinTech Group, Inc.), zonula occludens-1 (ZO-1; 1:200; cat. no. ab216880; Abcam) and claudin-2 (1:200; cat. no. ab53032; Abcam). Subsequently, after three washes with PBS, the sections were incubated with HRP-conjugated

(14) Gender‐based effect of absence of gut microbiota on the protective efficacy of Bifidobacterium longum‐fermented rice bran diet against inflammation‐associated colon tumorigenesisMolecular CarcinogenesisOctober 1, 2022Robin Kumar, Akhilendra K. Maurya, Kristopher D. Parker, Rama Kant, Hend Ibrahim, Md. Imtiazul Kabir, Dileep Kumar, Annika M. Weber, Rajesh Agarwal, Kristine A. Kuhn, et al.RRID:AB_2847859), NF-κB (p65) (#ab16502, RRID:AB_443394), ZO-1 (#ab96587, RRID:AB_10680012), Claudin-2 (#ab53032, RRID:AB_869174) and Alcian Blue PAS Stain Kit (#ab245876) were from Abcam (Cambridge, MA). Antibody

(15) Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial functionAnimal nutrition (Zhongguo xu mu shou yi xue hui)September 1, 2022He L, Zhou X, Wu Z, Feng Y, Liu D, Li T, Yin Y.Abcam), E-cadherin (Proteintech), claudin-1 (#13995, Cell Signaling Technology, Boston, USA), claudin-2 (ab53032, Abcam), AMPKα1 (10929-2-AP, Proteintech), AMPKα2 (18167-1-AP, Proteintech), PGC-1α (ab106814, Abcam),

(16) Improvement of the bladder perfusion curative effect through tight junction protein degradation induced by magnetic temperature-sensitive hydrogelsFrontiers in Bioengineering and BiotechnologyAugust 4, 2022Xiaoliang Sun, Xinhong Song, Peng Guo, Dong Zhang, Shishuai Zuo, Kang Leng, Yun Liu, Haiyang Zhanganti-OCLN (ab216327), anti-ZO-1 (ab276131), anti-JAMA (ab52647), anti-CLDN1 (ab211737), anti-CLDN2 (ab53032), and anti-CLDN4 (ab210796) antibodies were obtained from Abcam (Shanghai) Trading Co., LTD. (Shanghai,

(17) Role of Autophagy Inducers and Inhibitors in Intestinal Barrier Injury Induced by Intestinal Ischemia–Reperfusion (I/R)Journal of Immunology ResearchJuly 30, 2022Yuejin Li, Peng Zhang, Jian Zhang, Weimin Bao, Jinyuan Li, Yan Wei, Jinchao Ni, Kunmei GongAbcam, UK), anti-occludin (ab216327; Abcam, UK), anti-claudin-1 (ab180158; Abcam, UK), anti-claudin-2 (ab53032; Abcam, UK), anti-claudin-3 (ab52231; Abcam, UK), anti-Bcl-2 (ab182858; Abcam, UK), anti-Bax (ab32503;

(18) BATF3 Protects Against Metabolic Syndrome and Maintains Intestinal Epithelial HomeostasisFrontiers in ImmunologyJune 22, 2022Hussein Hamade et al.(Thermo Fisher Scientific, 40-4700), ZO-1 (Thermo Fisher Scientific, 61-7300), or Claudin-2 (Abcam, ab53032) overnight. Fluorophore-conjugated secondary antibodies were incubated for 1 h. Images were captured

(19) Medicinal Formula Huazhi-Rougan Attenuates Non-Alcoholic Steatohepatitis Through Enhancing Fecal Bile Acid Excretion in MiceFrontiers in PharmacologyJune 1, 2022Chunlin Li, Siyu Yu, Xiaoxiao Li, Ying Cao, Meng Li, Guang Ji, Li ZhangStates), ZO-1 (ab216880, Abcam, United States), Occludin (ab 216,327, Abcam, United States), Claudin 2 (ab53032, Abcam, United States), P-P65 rabbit monoclonal antibody (3031S, CST, United States), P65 rabbit monoclonal

(20) Interleukin-27 Regulates the Function of the Gastrointestinal Epithelial Barrier in a Human Tissue-Derived Organoid ModelBiology (Basel)March 11, 2022Daniel P. Brice, Graeme I. Murray, Heather M. Wilson, Ross J. Porter, Susan Berry, Scott K. Durum, Mairi H. McLeanIgG1, 1:5000, Abcam (ab8245)) protein expression. Proteins assessed were claudin-2 (rabbit IgG, 1:500, ab53032), claudin-4 (rabbit IgG, 1:1000, ab210796), E-cadherin (rabbit IgG, 1:10,000, ab40772) and occludin

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Anti-Claudin 2 antibody from Abcam

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Anti-Claudin 2 antibody from Abcam

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